Does this look like proper tetramer staining to you?
I cultured A2- lymph node cells with A2+ DCs and I want to read out if the LN T cells start responding to the DCs. I stained the cells with the same tetramer-cmv complex but two different fluorophores. I was hoping to get a clear double positive population but instead I got this. Does this look right to you?
Posted Dec 7, 2025, 11:45 PM UTC
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