Is there a perceptible difference between s24le vs s32le?

I have just a normal pair of headphones/headset, but I plugged it in to the motherboard on the back of my pc and I read s32le while using the dongle I get s24le I feel as though I can hear a difference, but it might be placebo, so I'm here to ask is it placebo or is there actually an audible difference for human ears?