Help with platinum run - man without equal

Hi friends, I’m attempting to platinum Sekiro and need some help with the man without equal trophy. I believe I’ve gotten all the bosses on one save file, but apparently not because I did not get my trophy.

Here are the bosses I’ve defeated on this save file. Could you please take a look and let me know if I’ve missed anyone?

SPOILERS AHEAD

• gyoubu
• Lady butterfly
• genichiro
• monkeys
• guardian ape
• headless ape
• corrupted monk
• true monk
• great shinobi owl
• owl (hirata)
• divine dragon
• Emma
• Isshin
• genichiro, way of tomoe
• isshin, sword saint
• demon of hatred

Did I miss any???

Only thing I can think of is I did not kill the serpent or the carp on this save file. Is that necessary?