Can I pay off some of my balance early to lower credit utilization?

I have a $200 limit card and a $100 limit card; I put all my subscriptions ($35 monthly) onto the $100 card. I just found out that is too much utilization, am I able to pay this $35 or pay half now and let auto pay finish the rest to lower the utilization?

Do they just measure the % throughout the month or just on/after the due date?