Urgent: Looking for Acetylcholinesterase (AChE) enzyme for thesis experiment (vial exchange or small aliquot)
I’m a graduating student from DLSMHSI. I’m reaching out in case any lab in the community might be able to help us with a small amount of Acetylcholinesterase (AChE) for an urgent phase of our thesis work.
We currently have Acetylcholinesterase from Electrophorus electricus (500 U/mg) on order, but unfortunately there has been an unexpected shipping delay, which means we can’t proceed with our scheduled HPTLC enzyme bioautography and microplate assays.
For our experiments, we need a total of ~30.225 Units (U) of AChE activity.
We were wondering if any lab might be able to help through one of the following options:
- Vial Exchange
If you have an unopened refrigerated vial of AChE (500 U/mg), we would be very grateful to borrow it. We will replace it with our brand-new identical vial once our shipment arrives (expected around April 2026).
- Small Liquid Aliquot
If a full vial isn’t available, would it be possible to provide a liquid aliquot of ~50–100 Units?
Our protocol uses enzyme reconstituted in 0.1 M phosphate buffer (pH 8.0). If your stock is in a different buffer or contains stabilizers, we can check compatibility with our TLC spray and assay reagents.
We’re happy to acknowledge your assistance in our thesis or compensate for reagent costs.
Thank you very much to anyone who might be able to help. Please feel free to comment or DM if you’re open to assisting.
Posted Mar 10, 2026, 11:39 AM UTC