Inspection Consequences

I am new to beekeeping by about a month with two hives located in suburban Pittsburgh, PA. Currently with one brood box each approaching 80% capacity across 9 frames.

The last inspection it was 50 degrees and drizzle. During, despite always best efforts and patience, I am concerned that some workers are getting crushed by the insertion/ removal of the frames. Post-inspection, a number of bodies were carried out and stacked on the porch, not dropped elsewhere. I am sure that each factor contributed to an agitated hive.

What I’d like to hear about is what’s a normal volume of unnatural loss during inspection? Either crushed by the lid or a frame movement. And how do we address concerns about a queen being harmed during inspection. Seems like I get a lot comb connecting frames too.

Of course inspection is a huge disruption for the hive, but should it also be a mass casualty event?