Title: How to Reconstitute Peptides the Right Way Dosing, Storage, and What Not to Do
This comes up all the time with people just getting started but that's ok. Mess this step up and you either waste the vial or end up with something that does nothing. Here is everything you need.
What you need
- 29 to 31 gauge insulin syringes (100 unit / 1mL)
- BAC water (bacteriostatic water)
- Alcohol wipes
- Sharps container
- Your peptide vial
How to do it
- Let the vial warm up to room temp before you touch it
- Wipe both rubber stoppers with an alcohol wipe, give them a few seconds to dry
- Draw a small amount of air into your syringe and push it into each vial first — both vials are under vacuum and this makes drawing the liquid out way easier
- Draw your BAC water into the syringe
- Push the water in slowly along the inside wall of the vial — never straight onto the powder
- Swirl it slow and gentle for about 30 to 60 seconds. Do not shake it
- Hold it up to light should be completely clear. Cloudy or particles floating around, toss it
- Write the date on the vial and get it in the fridge right away
Concentration calculator
| Vial Size | BAC Water | Concentration | 10 units on syringe = |
|---|---|---|---|
| 2mg | 2mL | 1,000 mcg/mL | 100 mcg |
| 5mg | 2mL | 2,500 mcg/mL | 250 mcg |
| 5mg | 5mL | 1,000 mcg/mL | 100 mcg |
| 10mg | 2mL | 5,000 mcg/mL | 500 mcg |
| 10mg | 10mL | 1,000 mcg/mL | 100 mcg |
When BAC water is the wrong choice
| Compound | What to use instead | Reason |
|---|---|---|
| IGF-1 LR3 / IGF-1 DES | 0.6% acetic acid | Clumps up at neutral pH |
| SLU-PP-332 | Dry capsule only | Will not dissolve in water |
| Intranasal peptides | Bacteriostatic saline | Less irritating on nasal tissue |
| Glutathione | BAC water but use it same day | Breaks down within 24-48 hours |
Storage
| Compound | Unopened | After mixing |
|---|---|---|
| Most peptides | 2-8°C up to 24 months | 2-8°C up to 28 days |
| GLP-1s | 2-8°C | Do not freeze once mixed |
| Glutathione | Keep away from light | Same day use only |
| Melanotan II | 2-8°C in foil | 28 days, keep it covered |
| HCG | 2-8°C | 30-60 days, freezing destroys it |
| Nasal sprays | 2-8°C | 14-30 days once opened |
Common mistakes worth avoiding
- Shooting BAC water straight onto the powder instead of the side wall
- Skipping the air step and fighting with the vacuum trying to draw liquid
- Shaking the vial — swirl only, always
- Leaving reconstituted peptides sitting out at room temp
- Freezing a vial after it's already been mixed
- Forgetting to date the vial when you mix it
- Grabbing BAC saline instead of BAC water different products, easy mix up
Bookmark this if you are just getting into peptides. These are the basics that trip people up the most.
Any questions on a specific compound or dose drop them below.
Not a doctor. Just someone who reads too much PubMed at midnight.
Posted Jun 2, 2026, 1:04 PM UTC
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