Mouse IL-6 and GM-CSF staining with Foxp3 kit

I love this table from Thermo but unfortunately they don't list or have tested mouse IL-6 or GM-CSF staining. Anyone know if the Foxp3 kit is compatible with staining mouse IL-6 and GM-CSF?

For details general work flow is: in vivo mouse model that's very inflammatory, pulling out spleen and processing to single cell suspension, will be doing 4hr PMA Iono stim, would ideally like to use the Foxp3 kit bc I have a couple of intranuclear targets and would ideally not have to split into a separate panel using CytoFix/Perm. But if anyone has experience with staining for either of the targets above with the nuclear kit, would love to hear how it went.

Thanks!

https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-analysis-learning-center/cell-analysis-resource-library/ebioscience-resources/intracellular-staining-buffer-selection-guide.html

Posted Aug 5, 2026, 1:37 PM UTC

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