Yubikey on keepassXC

A couple of years I have opened my .kdbx db on keepassXC with master pw , keyfile and yebikey. Now I want to configure a now secret on my challenge-response. Therfore I remove challenge-response in keepassxc db settings.

When I now try to open the .kdbx db w master pw and keyfile I get the red upper message: db could not be loaded or something.

When I try to open db with master pw,keyfile and yubikey ( old secret): again red message.

Is reco´figuratin challeng-response not stable in keepassXC?