Bagging media during incubation

I work in a manufacturing lab for a medical device/ pharma company. Our environmental monitoring plates were having an issue drying out in the incubator (30-35C) when incubated 6 or 7 days. They must incubate at least 5 days, but plates incubated on Monday and Tuesday of any week will remain in the incubator until the following Monday (so, 6/7 days incubation). To remedy the dry plate issue, my manager decreed that all plates being incubated for ANY length of time should be bagged in a sealed bag (think, 2 gallon Ziploc bags). But now we are having issues with excessive moisture on growth promotion plates, which are incubated for less than 72 hours.
What I have been witnessing is that bags of media incubated for less than 3 days come out visibly wet and holding moisture in the lid that flows across my colonies when I flip the plates to debag or read them. In contrast, bags incubated over 5 days come out of the incubator dry.
My manager insists that there is no way that bags can have moisture in them at 3 days and then not have moisture at 5 days. My assumption is that the moisture first evaporates from the plates into the bags, then is reabsorbed into the media as the media dries out. Or the moisture evaporates into the bags and over longer time periods, the moisture is lost through the bag.

He also insists that bagging all media plates is "industry standard" and every company that knows what they are doing is bagging all media during incubation. He is basing that on the fact that Thermo Fisher (where he used to work) used to include a separate zippered bag inside the packaging of their double and triple wrapped media, meant to hold the plates during incubation. I thought those extra bags were to be able to "reseal" the leftover plates after you open a sleeve of media. but, of course, I am the idiot here, so what do I know.

Has anyone else had this issue and how did you overcome it? I will need sources to back up whatever I say to him to justify a different route. If it matters, all plates in this discussion are TSA and TSA contact with L & P80.

Posted Aug 13, 2026, 4:38 PM UTC

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