Does splicing machinery "expect" introns to be a certain size?

I have no idea if this is a real thing and can't figure out the right keywords for searching!

Basically imagine Gene A has an intron that is 5kb and Gene B has one that's 100bp.

If I inserted a 1kb sequence (without splice sites) into each intron, would this be expected to affect expression? And if so, would we expect Gene B to be affected more, because the size of the intron increased more proportionally?

Posted Aug 31, 2026, 2:31 AM UTC

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