But if I just went and killed them all, that would affect my alignment, right?  I think I failed on a previous wiz quest because I killed dozens of aligned gremlins that were multiplying out of control.

Monsters killed by other monsters, including pets, do not count as kills for a the purpose of alignment record. This is why pacifists sometimes have trouble improving their alignment record.

Am I going to mess up my alignment if I kill off a room full of peaceful monsters?  I'm creating monsters and sacrificing them, and the room is filling with annoying monsters.  I think I ruined my alignment in another game by killing a bunch, but the wiki isn't clear.

I don't have a pet to kill them, but I do have a ring of conflict.  Does that count as a kill?

Killing peaceful monsters and alignment