r/biology•
xaqeri

Flow staining help!

I'm sure this will get downvoted into oblivion as this may be a strange post here. I'm staining some cell to run on my LSRII and I accidentally permeabilized after I stained with my primary but before I added my secondary. If I stain with the secondary now, before adding my IC stain, is there a chance for non-specific intracellular staining? Am I screwed in terms of detection of that primary?

Posted Dec 2, 2011, 2:40 AM UTC

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