Very small guy I cannot identify
Posted Apr 8, 2022, 8:15 AM UTC
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Looks like a handsome little amoeba! Probably feeding on your bacterial culture.
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DM me for some primer sequences that work on Amoeba 18S rDNA if you want to try them!
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Wow, thanks. Can they really be that small?
I actually might have some primers, I just didn't know I needed them. But thank you very much. I'll maybe get back to you on that.
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It’s hard to tell just based on this video! You’re talking about the amorphous blob center-stage, right, and not the vibrating bacteria? Was it moving at all?
I think the size is possible! Amoebae can be pretty tiny depending on the species and stage of life.
I have loads of Vermamoeba in my algae samples and they are impossible to get rid of.
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It is actually the vibrating bacteria I'm talking about. They don't return any sequence.
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Ah! Lol the dust looks very much like an amoeba, then!
Are you checking your amplicons on a gel before sending? Are you using Sanger sequencing? You could try trimming your sequencing primer a bit from the 3’ end (should end with a C or G) or if you aren’t even getting amplification, lower your PCR annealing temp by 3 degree increments down to 9-10 degrees lower. You could also try sequencing with your reverse primer.
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Sorry for the shitty video.
This is with 1000x magnification, so it is very small. I'm having a hard time identifying it.
I tried culturing what I thought was a dead Leptospira broomi culture, and this came out. It does not look like Leptospira to me, but I might be wrong?
I tried sequencing it and it came up as Micrococcus luteus, which I have later found as contamination in the medium. But no other sequences came up, so I couldn't even BLAST it.
Does anyone have the faintest idea?
It is from liquid Leptospira EMJH culture medium.
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Vitamin c