How to do statistics for quantitation of fluorescence?

Hi all! Im doing analysis of intensity of a feature in cells (lipid peroxidation). My cells are a mixed cell culture of brain cells (glial cells and neurons), and it’s impossible to get the intensity per cell. So what I do is get the intensity per field of view, and normalize by the number of cells. I’m comparing untreated and treated cells. But how should I run the statistics on this kind of data? I ran a t-test with each point being one field of view. But a labmate said I shouldn’t do that, because “of course I’ll get statistical significance, since I had so many data points”. Someone else said I should average the intensity of field of views of the same well, then plot the averages.

Do you guys have any insights on how I could do that? Thank you!