Making a Twilight D&D one-shot adventure for my wife and friends. Help!

For her birthday, my wife asked me to run a D&D one-shot game for her and her friends, set in the world of Twilight. I'm comfortable running D&D, but I know nothing at all about Twilight. I'll watch all the movies and do whatever research I can to understand the lore, but what I really need help with is this:

If you were playing in a Twilight D&D session, what would make it feel like Twilight to you? What are the core themes, emotions, conflicts, and tropes you would expect to make it feel like Twilight? Beyond these specific questions, if you have any other advice or insights, I'm all ears.