AmazingUsual3045

Hi all,

Hoping to get a bit of help with the SP6 mMessage mMachine kit. I’ve been making mRNAs with this kit for micro injections, and it’s been going fine, I get expression off of the transcripts I inject, but I’m unhappy with the quality of the mRNA I produce. Some transcripts give perfect clean slim peaks by tapestation, but others I get real broad peaks. Same data but by gel, I’ll get some with clean bands with just a dusting of smear below it, but some band is barely visible with just a smear. Same result when I use the kit control template. Both express fine, but I’d love to be injecting as a pure a product as possible. I’ve worked with rna a lot, I’m RNA zapping everything in site,

Hi All,
My mom is a graphics designer, and has been an artist professionally and recreationally her whole life. Recently, she’s started to explore water color and is pretty into it. Her birthday is coming up, and I wanted to get her something related to her new passion but I’m not sure what to get; she’s been an artist for a long time and has all the niche equipment. Is there any water color specialty items anyone can recommend?

I’m working on an A350T with the dual printing head. I’ve finally tuned in my PVA really well and it prints perfectly. My problem is I’m having so much trouble getting the PLA to actually stick to the PVA (PVA and PLA are both snapmaker brand). I’ve been printing with PLA for a decade and I’ve never encountered so much trouble with it, it usually prints and sticks really well. Bed is heated to 65c, I’ve tried multiple snapmaker colors, all with the same problem.

Thanks for any help!

Hi!

I’m looking for an anime to watch that follows characters or a culture through time and you get to see society evolve technologically or biologically. Ideally something finished.

I’m thinking things like:

- Vivy

- Dr. Stone

- Rin: daughters of Mnemosyne

Thanks!

So it’s around the months before Christoper dies, Phil is being a hard ass. Let’s say you’re Tony, what’s your strategy to actually appease Phil and get back in good graces with NY? Is it even possible? Would Phil ever get over his brother (whatever happened there) or did Tony kinda doom himself to Phil’s ire after he shot Blundetto?

Hi All,

Has anyone trained with any of the new(ish) sokes of gikan, kumogakure, or gyokushin ryu? What are these ryu like? Been in bujinkan for awhile now and I’ve seen a technique/principle here and there from these schools, but nothing really unifying. I was curious if these ryu will be more prominent now that they have specific people to disseminate them.

Hi all,

Used to be a daily pot smoker and when I would roll I would smoke ~90min in and during the come down. I quit pot this year and haven’t smoked for 8ish months. I’m gonna roll in the next couple days without pot been a year since my last roll, I’m curious, how will this roll be different? What should I expect?

Thanks!

Hi All,

Sorta a silly question. My toddler loves flat cookies shaped like animals but is horrified and disgusted at the thought of eating a broken one😱. Is there an edible glue out there that would work to stick cookie pieces back together?

Hi,

My wife and I are struggling in our marriage largely from things that I have done. We’ve both agreed amends need to be made for the past, but my wife is having a lot of trouble verbalizing what she needs concretely as an amends. She’s able to reject ideas I come up with, so she knows what feels wrong, but we’re having trouble finding what’s right. She likes the example of, if you hit someone’s car, not only do you pay to repair it but you pay associated fees (car rental, etc.) and you develop routines to not hit other cars again.

The problem I’m having, is how to I make amends for things that didn’t cause financial damage. For example I drank very heavily for 6mo. No abuse, no damage t

Hi,

My wife and I are struggling in our marriage largely from things that I have done. We’ve both agreed amends need to be made for the past, but my wife is having a lot of trouble verbalizing what she needs concretely as an amends. She’s able to reject ideas I come up with, so she knows what feels wrong, but we’re having trouble finding what’s right. She likes the example of, if you hit someone’s car, not only do you pay to repair it but you pay associated fees (car rental, etc.) and you develop routines to not hit other cars again.

The problem I’m having, is how to I make amends for things that didn’t cause financial damage. For example I drank very heavily for 6mo. No abuse, no damage t

Ok hear me out, I know this is the opposite of what you’d expect. Smoked everyday from 23 - 38, but I’ve been sober the last 2.5months. I somehow had a pretty decent memory during my many stoner years, but I feel like my memory has decreased terribly since I sobered up. Anyone else? Is this a thing? It feels pretty weird to have worse memory now.

Hi All,

I’m finishing up my PhD in biology (synthetic biology / virology) and oh boy am I burned out. Like F science burnt out. I’ve loved science and biology my whole life, and I feel like I’m losing a kinda critical part of what makes me me. Some of it I think is it’s been a long PhD (7years!) and some of it is that my advisor kinda abandoned me after my committee took my side over something. Do I just need to spend some time after defending to kinda be in a calmer place? Anyone else gone through this kinda thing? How did you deal with burn out?

Thanks for any help it would be greatly appreciated!