Darth_insomniac•

Pathologist here. Here you go

It does look like an elastin stain. The fibers should stain black, and it looks like it does in a small artery.

Usually tissue is stained with Hematoxylin and Eosin, which highlights the cellular details better.

Hope you're having fun in your histology class! :)

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knowone572OPastrobiology•

Thank you for the analysis! Once my peers have had a look at the original I'll share this with the class.

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Darth_insomniac•

No problem :)

I'm pretty sure that it's a small artery, but I couldn't definitely identify the lumen. Can't think of what other skin structure it could be that would have that shape and pick up the black coloration. I think it's probably just overstained.

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all_seeing_ey3bio enthusiast•

You should hood yourself up with pathologist flair!

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lumometer•

Thank god, thank you. Only took an intro histo course and it was driving me nuts. Makes sense now...

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acdurkes•

Hi! I'm a vet pathologist. I'm going to save your comment so I can bug you about weird cases that need an MD perspective.

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ailbolesmolecular biology•

That's what I thought it was but couldn't be sure because its been years. Thanks for the analysis!

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[deleted]•

Another A&P TA and I are stumped; what is this?

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EColi452•

That sir, is a cross section.

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[deleted]•

Oh, you. I meant what is it a section of.

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EColi452•

I assumed you were, alas i am at a loss for we just started histology in my vertebrate anatomy course. Sorry about that, surely OP will deliver.

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knowone572OPastrobiology•

Don't hold your breath :)

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[deleted]•

Brilliant analysis!

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Sybertron•

Sure looks like it may contain lots of the common elements in a "tissue specimen" as well. We'll need more testing for further analysis.

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knowone572OPastrobiology•

I took this photo during the Connective Tissue lab. I'm mad at myself for not recording which slide it was from, since that's something I normally do. I remember that I basically skipped over the slide because I couldn't even remotely find the structures that I was looking for. I'm thinking that I was supposed to be looking for elastic connective tissue in this one, but I'm not sure. I have lab again on Wednesday, I think I had either slide set 8 or 9, so I'll go over some of those again and get back to you.

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[deleted]•

Much obliged.

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AlaskanPotatoSlap•

Correct me if I'm wrong, but the ECT is cutting diagonally through the top right corner. The thick purple line that is medial to what looks to be dense connective tissue. I've taken Basic A&P, and currently in A&P 1, so I could be and probably am wrong.

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knowone572OPastrobiology•

Sounds plausible. I'm going to grill my TA and his assistants about it too. If all else fails I'll bring it to my professor. Surely, OP will deliver on this one.

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yaleski•

I believe it is a cross section of an ovary.

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YouHaveGonorrhoeae•

That was my first instinct too. But I'm a microbiologist. What the hell do I know? ;)

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[deleted]•

Mine, too, but my female (and very knowledgeable co-TA says no way -- we're still stumped, though.) She also thought it was stratified squamous with keratin on the top right, but it's too thin to be skin... Hmm...

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thatsdrinkin•

looks like a muscle cs in the bottom left. maybe mesenchyme in the middle

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Flounderfindertoxicology•

I think you're right.

I haven't done this in years but it looks like there is skeletal muscle cells in the bottom left, mesenchyme in the middle and a couple of arteries. The arteries make up the eyes of the 'scream.' There is skin (squamous cells) in the top right.

OP was looking for elastic connective tissue. I would expect as a ring around each artery (I can't recall if it would be the outermost or innermost layer).

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thatsdrinkin•

i think the eyes might be teeth

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Flounderfindertoxicology•

Yeah, I saw circles and thought arteries. The circles in the middle look like bubbles from the fixation. but, I don't know what teeth in cross section look like.

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tchomptchompdevelopmental biology•

I feel like there are some muscle cells in the bottom righthand corner. The block in the middle might be hyaline cartilage, perhaps?

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mcacphysiology•

I'm an A&P instructor and I too am stumped. I'm not sure what the circular structures are supposed to be - they don't look like blood vessels.

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muyhairyballz•

creepy

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Stormygeddon•

It is bound to happen with random patterns. But yeah, that is rather disturbing.

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expathaligonian•

Brings me back...I loved Histology. Could spend hours looking through microscopes. My favourite area was bone development.

How'd you do on the exam? Enjoying the class?

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WilsonElement154•

I'm only an undergrad genetics student so when I saw this title pop up I assumed you'd been indoctrinated by some some sort of scientologist "re-education" program.

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all_seeing_ey3bio enthusiast•

I know that feel. Histo had the most brutal exams I ever took...

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StayinFrosty•

awesome shit man "1 point to histology" lol

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knowone572OPastrobiology•

You just joining us, or are you too afraid to use your real account? :)

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